ABSTRACT
OBJECTIVES: The relationship between adenosine deaminase and various cancers has been investigated in several studies. However, serum adenosine deaminase activity and carbonic anhydrase and catalase activities in patients with bladder cancer have not previously been reported. Therefore, the aim of this study was to measure serum adenosine deaminase, carbonic anhydrase and catalase activities in patients with bladder cancer. MATERIALS AND METHODS: Forty patients with bladder cancer and 30 healthy controls were enrolled in the study. Serum adenosine deaminase, carbonic anhydrase and catalase activities were measured spectrophotometrically. RESULTS: Serum adenosine deaminase, carbonic anhydrase and catalase activities were significantly higher in patients with bladder cancer than controls (all significant, p<0.001). CONCLUSIONS: These markers might be a potentially important finding as an additional diagnostic biochemical tool for bladder cancer.
Subject(s)
Aged , Humans , Male , Middle Aged , Adenosine Deaminase/blood , Carbonic Anhydrases/blood , Catalase/blood , Biomarkers, Tumor/blood , Urinary Bladder Neoplasms/enzymology , Epidemiologic Methods , Spectrophotometry , Urinary Bladder Neoplasms/bloodABSTRACT
Red blood cell carbonic anhydrase (CA) activity has not been studied in high altitude natives. Because CA is an intraerythocytic enzyme and high altitude natives are polycythemic, it is important to know if the activity of CA per red cell volume is different from that of their sea level counterparts. Blood was collected from healthy subjects living in Lima (150m) and from twelve subjects from Cerro de Pasco (4330m), and hematocrit and carbonic anhydrase activity were measured. As expected, the high altitude natives had significantly higher hematocrits than the sea level controls (p = 0.0002). No difference in the CA activity per milliliter of red cells was found between the two populations. There was no correlation between the hematocrit and CA activity.
Subject(s)
Humans , Middle Aged , Altitude , Carbonic Anhydrases/blood , Erythrocytes , Erythrocytes/enzymology , Carbonic Anhydrases/metabolism , HematocritABSTRACT
Carbonic anhydrase enzyme activity could be measured by manometeric and colorimetric techniques. A simpler and modified method of carbonic anhydrase enzyme activity assessment in blood is proposed. In the present method differences in pH by hydration of CO2 in absence and presence of carbonic anhydrase inhibitor have been used to measure the carbonic anhydrase activity in the blood.
Subject(s)
Blood Chemical Analysis/instrumentation , Carbon Dioxide , Carbonic Anhydrase Inhibitors , Carbonic Anhydrases/blood , Humans , Hydrogen-Ion ConcentrationABSTRACT
Se determinaron los valores normales de actividad enzimática de anhidrasa carbónica eritrocitaria en adultos (X ñ 1 DE: 6546,4 ñ 1272,4 U/10 a la 7 hematíes), recién nacidos a término (X ñ 1 DE: 1694,8 ñ 1092,9 U/10 a la 7 hematíes) y prematuros (menos de 34 semanas X ñ 1 DE: 1025 ñ 873,2 y más de 34 semanas X ñ 1 DE: 1420,6 ñ 471,3), así como el comportamiento evolutivo en recién nacidos y prematuros de distintas edades gestacionales. Los prematuros con síndrome de distress respiratorio al nacer presentaron valores de anhidrasa carbónica muy bajos en comparación con los obtenidos en los grupos controles, por lo que se sugiere que la determinación de anhidrasa carbónica es útil para el diagnóstico de esta enfermedad
Subject(s)
Infant, Newborn , Adult , Humans , Carbonic Anhydrases/blood , Erythrocytes/enzymology , Respiratory Distress Syndrome, Newborn/enzymology , Respiratory Distress Syndrome, Newborn/bloodSubject(s)
Animals , Buffaloes/blood , Carbonic Anhydrases/blood , Drug Stability , Erythrocytes/enzymology , Isoenzymes/blood , Kinetics , Protein DenaturationABSTRACT
Foram estudadas as variaçöes ocorridas com as atividades enzimáticas do ácido delta-aminolevulínico desidratase, e da anidrase carbônica eritrocitária, em grupos de operários. Determinou-se que operários expostos, ocupacionalmente, ao chumbo, apresentam, além da diminuiçäo da atividade da ALA-D, também a diminuiçäo da atividade da anidrase carbônica
Subject(s)
Adult , Middle Aged , Humans , Carbonic Anhydrases/blood , Lead/pharmacology , Occupational Exposure , Porphobilinogen Synthase/bloodABSTRACT
A atividade da anidrase carbônica eritrocitária foi medida em três grupos de indivíduos: um näo fumante, outro fumante moderado e outro fumante imoderado. Os valores obtidos encontram-se significativamente aumentados no grupo fumante em comparaçäo aos abstêmios mesmo no subgrupo de fumantes moderados. A intensidade do hábito de fumar parece näo influir nos valores da anidrase carbônica entre os tabagistas. Valores do hematócrito e da hemoglobina encontram-se significativamente aumentados no grupo fumante